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human lung carcinoma a 549 cells  (ATCC)


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    ATCC human lung carcinoma a 549 cells
    Human Lung Carcinoma A 549 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 31888 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+lung+carcinoma+a+549+cells/A549/pmc13087728-217-0-5
    Average 99 stars, based on 31888 article reviews
    human lung carcinoma a 549 cells - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Virus:

    Article Title: Effect of Exposure to UV-C Irradiation and Monochloramine on Adenovirus Serotype 2 Early Protein Expression and DNA Replication
    Article Snippet: Human adenovirus serotype 2 strain adenoid 6 (VR-846; ATCC, Manassas, VA), was used as a model virus for this study (38). .. The virus stock was propagated in human lung carcinoma A-549 cells (CCL-185; ATCC, Manassas, VA) before use in experiments according to the following protocol, with the exception of experiment UV-1 using the virus stock directly upon shipment. ..

    Article Title: Effect of Exposure to UV-C Irradiation and Monochloramine on Adenovirus Serotype 2 Early Protein Expression and DNA Replication
    Article Snippet: Human adenovirus serotype 2 strain adenoid 6 (VR-846; ATCC, Manassas, VA), was used as a model virus for this study ( 38 ). .. The virus stock was propagated in human lung carcinoma A-549 cells (CCL-185; ATCC, Manassas, VA) before use in experiments according to the following protocol, with the exception of experiment UV-1 using the virus stock directly upon shipment. ..

    Article Title: Capsid-Damaging Effects of UV Irradiation as Measured by Quantitative PCR Coupled with Ethidium Monoazide Treatment.
    Article Snippet: The damage to a viral capsid after low-pressure (LP) and medium-pressure (MP) UV irradiation was assessed, using the quantitative or quantitative reverse transcription PCR coupled with ethidium monoazide treatment (EMA-PCR).. After UV irradiation, adenovirus 5 (Ad5) and poliovirus 1 (PV1) were subjected to a plaque assay, PCR, and EMA-PCR to investigate the effect of UV irradiation on viral infectivity, genome damage, and capsid damage, respectively.. The effectiveness of UV wavelengths in a viral genome and capsid damage of both PV1 and Ad5 was also further investigated using a band-pass filter.

    Cell Culture:

    Article Title: Triterpenoid derivatives inhibit Gli-mediated transcription in human glioblastoma cell line via direct interaction with Gli1.
    Article Snippet: .. Cell lines, chemicals, and antibodies Human glioblastoma U-87MG cells (HTB-14), human lung carcinoma A-549 cells (CCL-185), and mouse embryonic fibroblast NIH 3T3 cells (CRL-1658) were purchased from the American Type Culture Collection (ATCC, Manassas, USA) and cultured in DMEM medium (Sigma-Aldrich) supplemented with 10 % of FCS (SigmaAldrich) in a CO2 incubator at 37 °C. .. For the cell-based reporter assay, U-87MG cells Jo urn al Pr e-p roo f 18 were transferred to serum reduced OptiMEM medium (Invitrogen) supplemented with 0.5 % of FCS, 1 mM of sodium pyruvate (HyClone) and 1× concentrated non-essential amino acid solution (HyClone).

    Article Title: Triterpenoid derivatives inhibit Gli-mediated transcription in human glioblastoma cell line via direct interaction with Gli1
    Article Snippet: .. Human glioblastoma U-87MG cells (HTB-14), human lung carcinoma A-549 cells (CCL-185), and mouse embryonic fibroblast NIH 3T3 cells (CRL-1658) were purchased from the American Type Culture Collection (ATCC) and cultured in DMEM medium (Sigma-Aldrich) supplemented with 10% of FCS (Sigma-Aldrich) in a CO 2 incubator at 37 °C. .. For the cell-based reporter assay, U-87MG cells were transferred to serum reduced OptiMEM medium (Invitrogen) supplemented with 0.5% of FCS, 1 mM of sodium pyruvate (HyClone) and 1× concentrated non-essential amino acid solution (HyClone).

    Modification:

    Article Title: Inactivation kinetics of adenovirus serotype 2 with monochloramine.
    Article Snippet: The effect of pH (6–10), temperature (10–30 1C), disinfectant concentration (1–11 mg/l as Cl2), and ammonia nitrogen-to-chlorine molar ratio (1.3–52) on the inactivation kinetics of adenovirus serotype 2 with monochloramine was investigated by performing batch-reactor experiments with synthetic 0.01 M buffer (phosphate or borate) solutions.. The inactivation kinetics was independent of monochloramine concentration and ammonia nitrogen-tochlorine molar ratio but had strong pH dependence, with the rate of inactivation decreasing with increasing pH.. The kinetics at pH 6 and 8 were consistent with pseudo-first-order kinetics, while curves at pH 10 were characterized by a lag phase followed by a pseudo-firstorder phase.

    Infection:

    Article Title: Capsid-Damaging Effects of UV Irradiation as Measured by Quantitative PCR Coupled with Ethidium Monoazide Treatment.
    Article Snippet: The damage to a viral capsid after low-pressure (LP) and medium-pressure (MP) UV irradiation was assessed, using the quantitative or quantitative reverse transcription PCR coupled with ethidium monoazide treatment (EMA-PCR).. After UV irradiation, adenovirus 5 (Ad5) and poliovirus 1 (PV1) were subjected to a plaque assay, PCR, and EMA-PCR to investigate the effect of UV irradiation on viral infectivity, genome damage, and capsid damage, respectively.. The effectiveness of UV wavelengths in a viral genome and capsid damage of both PV1 and Ad5 was also further investigated using a band-pass filter.



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